| MD | Players | Clubs | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 27-Sep-2000 |  Andy Lonergan | Preston North End | England | 19-Oct-1983 | 16(344) |
| 19-Sep-2000 |  Kevin Braniff | Millwall | Northern Ireland | 04-Mar-1983 | 17(200) |
| 25-Sep-2000 |  Adam Hardy | Bradford City | England | 22-Feb-1983 | 17(216) |
| 26-Sep-2000 |  Jabo Ibehre | Leyton Orient | England | 28-Jan-1983 | 17(242) |
| 26-Sep-2000 |  Ben Clark | Sunderland | England | 23-Jan-1983 | 17(247) |
| 01-Nov-2000 |  Moritz Volz | Arsenal | Germany | 21-Jan-1983 | 17(285) |
| 01-Nov-2000 | Jermaine Pennant | Arsenal | England | 15-Jan-1983 | 17(291) |
| 20-Sep-2000 |  Simon Bryant | Bristol Rovers | England | 22-Nov-1982 | 17(303) |
| 20-Sep-2000 | Lewis Hogg | Bristol Rovers | England | 13-Sep-1982 | 18(8) |
| 19-Sep-2000 |  Stuart Parnaby | Middlesbrough | England | 19-Jul-1982 | 18(63) |
| 20-Sep-2000 | Benny McElholm | Leyton Orient | Northern Ireland | 07-Jul-1982 | 18(76) |
| 29-Nov-2000 |  Tony Crane | Sheffield Wednesday | England | 08-Sep-1982 | 18(83) |
| 20-Sep-2000 |  Lee Williamson | Mansfield Town | Jamaica | 07-Jun-1982 | 18(106) |
| 31-Oct-2000 |  Marc Richards | Blackburn Rovers | England | 08-Jul-1982 | 18(116) |
| 27-Sep-2000 |  Jay Bothroyd | Coventry City | England | 05-May-1982 | 18(146) |
| 27-Sep-2000 |  Mark Wright | Walsall | England | 24-Feb-1982 | 18(216) |
| 25-Sep-2000 | Scott Kerr | Bradford City | England | 11-Dec-1981 | 18(289) |
| 19-Sep-2000 |  Matthew Taylor | Luton Town | England | 27-Nov-1981 | 18(297) |
| 19-Sep-2000 |  Joe Cole | West Ham United | England | 08-Nov-1981 | 18(316) |
| 20-Sep-2000 |  Shaun Wright-Phillips | Manchester City | England | 25-Oct-1981 | 18(331) |
| 28-Nov-2000 |  Danny Webber | Manchester United | England | 28-Dec-1981 | 18(336) |
| 26-Sep-2000 | Simon Downer | Leyton Orient | England | 19-Oct-1981 | 18(343) |
| 26-Sep-2000 |  Ryan Ashford | Southampton | England | 13-Oct-1981 | 18(349) |
| 29-Nov-2000 | Owen Morrison | Sheffield Wednesday | Northern Ireland | 08-Dec-1981 | 18(357) |
| 26-Sep-2000 | Jay Murray | Leyton Orient | England | 23-Sep-1981 | 19(3) |
| 20-Sep-2000 | Richard Garcia | Leyton Orient | Australia | 04-Sep-1981 | 19(16) |
| 19-Sep-2000 | Tresor Kandol | Luton Town | Democratic Republic of Congo | 30-Aug-1981 | 19(20) |
| 01-Nov-2000 | Graham Barrett | Arsenal | Republic of Ireland | 06-Oct-1981 | 19(26) |
| 19-Sep-2000 |  Matthew Etherington | Tottenham Hotspur | England | 14-Aug-1981 | 19(36) |
| 19-Sep-2000 | Michael Carrick | West Ham United | England | 28-Jul-1981 | 19(53) |
| 26-Sep-2000 |  Titus Bramble | Ipswich Town | England | 21-Jul-1981 | 19(67) |
| 20-Sep-2000 | John Martin | Leyton Orient | England | 15-Jul-1981 | 19(67) |
| 31-Oct-2000 |  Joe Murphy | Tranmere Rovers | Republic of Ireland | 21-Aug-1981 | 19(71) |
| 20-Sep-2000 | Nathan Ellington | Bristol Rovers | England | 02-Jul-1981 | 19(80) |
| 25-Sep-2000 | Mark Kilty | Darlington | England | 24-Jun-1981 | 19(93) |
| 20-Sep-2000 |  Paul Konchesky | Charlton Athletic | England | 15-May-1981 | 19(128) |
| 19-Sep-2000 | George McCartney | Sunderland | Northern Ireland | 29-Apr-1981 | 19(143) |
| 26-Sep-2000 | Thomas Butler | Sunderland | Republic of Ireland | 25-Apr-1981 | 19(154) |
| 31-Oct-2000 | Paul Rachubka | Manchester United | England | 21-May-1981 | 19(163) |
| 26-Sep-2000 |  Michael Dobson | Brentford | England | 09-Apr-1981 | 19(170) |
| 01-Nov-2000 | Christopher Kirkland | Coventry City | England | 02-May-1981 | 19(183) |
| 31-Oct-2000 | John O'Shea | Manchester United | Republic of Ireland | 30-Apr-1981 | 19(184) |
| 19-Sep-2000 | Steven Reid | Millwall | Republic of Ireland | 10-Mar-1981 | 19(193) |
| 19-Sep-2000 | Tommy Tyne | Millwall | England | 02-Mar-1981 | 19(201) |
| 20-Sep-2000 | Charlie MacDonald | Charlton Athletic | England | 13-Feb-1981 | 19(219) |
| 19-Sep-2000 | Mark Maley | Sunderland | England | 26-Jan-1981 | 19(236) |
| 20-Sep-2000 |  Francis Jeffers | Everton | England | 25-Jan-1981 | 19(238) |
| 31-Oct-2000 | Michael Stewart | Manchester United | Scotland | 26-Feb-1981 | 19(247) |
| 01-Nov-2000 |  Gareth Barry | Aston Villa | England | 23-Feb-1981 | 19(251) |
| 28-Nov-2000 |  Mikael Forssell | Crystal Palace | Finland | 15-Mar-1981 | 19(258) |
MD = Match date,
DOB = Date Of Birth,
A(D) = Age(Days).