| MD | Players | Clubs | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 13-Aug-2016 |  Alphonso Davies | Vancouver Whitecaps | Canada | 02-Nov-2000 | 15(284) |
| 14-Apr-2016 | Tyler Adams | New York Red Bulls | United States | 14-Feb-1999 | 17(60) |
| 02-Jun-2016 | Ben Swanson | Columbus Crew | United States | 18-Jul-1997 | 18(320) |
| 20-Mar-2016 | Justen Glad | Real Salt Lake | United States | 28-Feb-1997 | 19(20) |
| 06-Mar-2016 | Tommy Redding | Orlando City | United States | 24-Jan-1997 | 19(41) |
| 06-Mar-2016 | Rodrigo Ramos | Chicago Fire | Brazil | 14-Jan-1997 | 19(51) |
| 24-Jul-2016 | Coy Craft | FC Dallas | United States | 23-May-1997 | 19(62) |
| 30-Apr-2016 | Collin Fernandez | Chicago Fire | Peru | 13-Feb-1997 | 19(76) |
| 06-Mar-2016 | Matheus Silva | San Jose Earthquakes | Brazil | 08-Dec-1996 | 19(88) |
| 02-Jun-2016 | Cristian Martinez | Columbus Crew | Panama | 06-Feb-1997 | 19(116) |
| 03-Apr-2016 | Bradford Jamieson | Los Angeles Galaxy | United States | 18-Nov-1996 | 19(136) |
| 21-May-2016 | Jack Harrison | New York City | England | 20-Nov-1996 | 19(182) |
| 06-Mar-2016 | Anatole Bertrand Abang | New York Red Bulls | Cameroon | 06-Jul-1996 | 19(243) |
| 23-Oct-2016 | Eric Ayuk Mbu | Philadelphia Union | Cameroon | 17-Feb-1997 | 19(248) |
| 25-Sep-2016 | Pablo Baez | Real Salt Lake | Paraguay | 15-Jan-1997 | 19(253) |
| 23-Oct-2016 | David Choiniere | Montreal Impact | Canada | 07-Feb-1997 | 19(258) |
| 13-Mar-2016 | Deybi Flores | Vancouver Whitecaps | Honduras | 16-Jun-1996 | 19(270) |
| 11-Sep-2016 | Derrick Etienne | New York Red Bulls | Haiti | 25-Nov-1996 | 19(290) |
| 27-Aug-2016 | Caleb Calvert | Colorado Rapids | United States | 22-Oct-1996 | 19(309) |
| 06-Mar-2016 | Kianz Froese | Vancouver Whitecaps | Canada | 16-Apr-1996 | 19(324) |
| 09-Apr-2016 | Marco Bustos | Vancouver Whitecaps | Canada | 22-Apr-1996 | 19(352) |
| 20-Mar-2016 | Zachary Herivaux | New England Revolution | Haiti | 01-Feb-1996 | 20(48) |
| 29-May-2016 | Jordan Hamilton | Toronto FC | Canada | 17-Mar-1996 | 20(73) |
| 29-May-2016 | Harrison Heath | Orlando City | England | 06-Mar-1996 | 20(84) |
| 07-Mar-2016 | Oalex Anderson | Seattle Sounders | Saint Vincent and the Grenadin | 11-Nov-1995 | 20(117) |
| 16-Oct-2016 | Yair Arboleda | Houston Dynamo | Colombia | 07-Apr-1996 | 20(192) |
| 10-Apr-2016 | Alex Muyl | New York Red Bulls | United States | 30-Sep-1995 | 20(193) |
| 06-Mar-2016 | Tommy Thompson | San Jose Earthquakes | United States | 15-Aug-1995 | 20(204) |
MD = Match date,
DOB = Date Of Birth,
A(D) = Age(Days).